Anti-hIST1/IST1 Antibody Picoband® A05507-2 from BosterBio

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Anti-hIST1/IST1 Antibody Picoband®

Western blot analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (A05507-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human MRC-5 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1/IST1 antigen affinity purified polyclonal antibody (Catalog # A05507-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1/IST1 at approximately 40 kDa. The expected band size for HIST1/IST1 is at 40 kDa.
Anti-hIST1/IST1 Antibody Picoband®

IHC analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (A05507-2).
HIST1/IST1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HIST1/IST1 Antibody (A05507-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Anti-hIST1/IST1 Antibody Picoband®

IHC analysis of HIST1/IST1 using anti-HIST1/IST1 antibody (A05507-2).
HIST1/IST1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HIST1/IST1 Antibody (A05507-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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BosterBio for
Anti-hIST1/IST1 Antibody Picoband®

The Anti-hIST1/IST1 Antibody Picoband® from BosterBio is a Rabbit Polyclonal antibody to IST1, and IST1 factor associated with ESCRT-III. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Anti-hIST1/IST1 Antibody Picoband® has been validated for the following applications: EIA, Immunoassay, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot.

Description

Boster Bio Anti-hIST1/IST1 Antibody Picoband™ catalog # A05507-2. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human, Mouse, Rat

Anti-hIST1/IST1 Antibody Picoband®

Anti-hIST1/IST1 Antibody Picoband®

Anti-hIST1/IST1 Antibody Picoband®

Anti-hIST1/IST1 Antibody Picoband® from BosterBio

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Anti-hIST1/IST1 Antibody Picoband® from BosterBio

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